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KMID : 0613820090190121802
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Journal of Life Science
2009 Volume.19 No. 12 p.1802 ~ p.1807
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Effects of Platycodin D on Gene Expressions of Pro-adipogenic and Anti-adipogenic Regulators in 3T3-L1 Cells
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Lee Hae-Yyong
Kang Ryun-Hwa
Cho Soo-Hyun
Kim Sung-Su
Kim Yeong-Shik
Yoon Yoo-Sik
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Abstract
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Platycodin D, a major component of Platycodi radix, is known to have various activities including anti-inflammatory, anti-hyperlipidemic, anti-tumor activities and others. Recently, it was reported that platycodin D inhibits fat accumulation and adipogenesis. The aim of this study was to investigate whether various adipogenic regulators are modulated by platycodin D treatment during the adipogenesis of 3T3-L1 cells. mRNA levels of terminal markers of adipogenesis such as ADIPOQ (adiponectin) and GLUT (glucose transporter) 4, which were quantified by real time PCR, were decreased by platycodin D treatment. mRNA expression of PPAR (peroxisome proliferator-activated receptor) γ and C/EBP (CCAAT/enhaner binding protein) α, which are central transcription factors of adipogenesis, were also decreased by platycodin D treatment. To elucidate the detailed molecular mechanism of platycodin D-induced inhibition of adipogenesis, we analyzed mRNA expression of upstream regulators of PPARγ and C/EPBα. mRNA levels of the pro-adipogenic regulators, KROX20 and KLF (Kruppel-like factor) 15 were markedly down-regulated by platycodin D treatment. On the other hand, mRNA expression of CHOP (C/EBP homologous protein), an anti-adipogenic regulator, was significantly up-regulated by platycodin D treatment. mRNA levels of other pro-adipogenic regulators, C/EBPβ and C/EPBδ, were not affected by platycodin D treatment, and another anti-adipogenic regulator, C/EBPγ was also not affected by platycodin D treatment. Taken together, these results suggest that platycodin D-induced inhibition of adipogenesis is mediated by gene interactions including the down-regulation of pro-adipogenic regulators, KROX20 and KLF15, and the up-regulation of an anti-adipogenic regulator, CHOP.
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KEYWORD
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Adipogenesis, platycodin D, 3T3-L1, KROX20, KLF15, CHOP
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